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Well, there actually seem to be a problem with libfluidsynth 1.1.1 re. playing MIDI files after all. Somehow it shutdowns the audio engine (eg. JACK) when it starts playing a MIDI file, like after you drag-and-drop one over the main Qsynth window.

However, there's this way for you to render audio from a MIDI file playback: you setup the audio driver to file and enter a filename (.wav) into the audio device name. From that moment all audio output will be written to that file instead of sent to soundcard/speakers/whatever it was before. Drag and drop the MIDI file for immediate playback and just wait (in silence) for the duration of the whole sequence. Then you can change back the audio driver to its previous setting. After all that you can open the resulting wav file into audacity and prepare for some editing or some listening and trimming of course.

HTH